Karyotype bone marrow
Includes culture processing charge
2 - 10 ml bone marrow aspirate in RPMI, Bone marrow trephine in RPMI
4 ml Heparin (Dark green) Whole blood sterile Do not centrifuge or freeze.
Blood Samples are preferred for CLL and ?LPN patients with peripheral lymphocytosis.
For other disorders, peripheral blood is suitable if there are >10% blasts in circulation.
Ambient on day of collection. Refrigerate overnight or weekend then send ambient
Urgent < 5 days, Non-urgent 18 days
Cell culture, arrest in metaphase, microscopic analysis
Detection and monitoring of acquired chromosome abnormalities from bone marrow in the diagnosis of leukaemia.
1 ml Bone marrow aspirate in RPMI