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Laboratory Information

Test Name

ANCA or MPO or PR3

Synonyms

Anti neutrophil cytoplasmic antibodies
Myeloperoxidase antibodies
Proteinase 3 antibodies

Specimen Collection

Adult Specimen

Gold or Red tube

Paediatric Specimen

Paediatric Gold or Red tube

Instructions for Referral to Waikato Lab

Aliquot instructions

500uL Serum

Aliquot Transport

Chilled

Test Information

Department

Immunology

Test Availability

Mon -Fri, 0800-1630

Laboratory Turnaround Time

48 hours

Method

MPO/PR3 - Chemiluminescence ANCA - Indirect immunofluorescence.

Uncertainty of measurement

MPO <20%, PR3 <30%

Diagnostic Use Or Instructions

The following are indications for an ANCA test (no exceptions):

Glomerulonephritis (especially rapidly progressive GN (RPGN),
Pulmonary haemorrhage (especially pulmonary renal syndrome),
Cutaneous vasculitis with systemic features, multiple lung nodules, and/or arthritis
Chronic destructive disease of the upper airways,
Longstanding sinusitis or otitis,
Subglottic tracheal stenosis,
Mononeuritis multiplex or other peripheral neuropathy,
Retro-orbital masses,
Monitoring response to treatment (more controversial).

Additional Information

MPO and PR3 antibodies only are tested in the first instance on all ANCA requests. Please notify the laboratory if clinical details suggest IBD/gastroenterology so ANCA IIF testing can be performed instead.
First time positive MPO/PR3 will be followed up with ANCA IIF testing on the next working day.

Laboratory Test Reference Guide