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Laboratory Test Reference Guide

Laboratory Information

Test Name

1,25 Dihydroxy Vitamin D


1,25 Vitamin D

Specimen Collection

Adult Specimen

Serum Gold Top

Test Information



Test Availability

Referred to external laboratory.

Diagnostic Use Or Instructions

Testing 1,25 Dihydroxy Vitamin D is rarely indicated. Indications include investigating unexplained hypercalcemia or inherited disorders of Vitamin D metabolism.

1,25 Dihydroxy Vitamin D has a short half-life. To aid interpretation of the 1,25 Dihydroxy Vitamin D result, PTH and calcium MUST be requested simultaneously with 1,25 Dihydroxy Vitamin D requests.

Additional Information

Testing for 1,25 Dihydroxy Vitamin D has an even more limited role.

Choosing Wisely note:

Do not routinely measure 1,25 Dihydroxy Vitamin D unless the patient has unexplained hypercalcemia or decreased kidney function.

Choosing Wisely add:

Many practitioners become confused when ordering a vitamin D test.

Because 1,25 Dihydroxy Vitamin D is the active form of vitamin D, many think that measuring 1,25 Dihydroxy Vitamin D is an accurate means to estimate vitamin D stores and test for vitamin D deficiency, which is incorrect.

Please note: 1,25 Dihydroxy Vitamin D is NOT available as a "paid test"

Referred Out

Electronic Information

Registration Code