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Laboratory Test Reference Guide

Laboratory Information

Test Name

1, 25 Vitamin D


Calcitriol, 1,25 dihydroxyvitamin D, 1.25 OH Vit D, 1.25 OH Vitamin D

Specimen Collection

Patient Specimen

Serum Gold Top

Test Information



Test Availability

Referred to external laboratory.

Diagnostic Use Or Instructions

Testing 1, 25 Vitamin D is rarely indicated. Indications include investigating unexplained hypercalcaemia or inherrited disorders of Vitamin D metabolism.

Additional Information

Testing for 1,25-hydroxy Vitamin D has an even more limited role.

Choosing Wisely note:

Do not routinely measure 1,25 dihydroxyvitamin D unless the patient has unexplained hypercalcemia or decreased kidney function.

Choosing Wisely add:

Many practitioners become confused when ordering a vitamin D test.

Because 1,25-dihydroxyvitamin D is the active form of vitamin D, many think that measuring 1,25-dihydroxyvitamin D is an accurate means to estimate vitamin D stores and test for vitamin D deficiency, which is incorrect.

Referred Out

 Queensland Health Pathology, Royal Brisbane Hospital

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Electronic Information

Registration Code